人LIGHT ELISA试剂盒(ab100583)

概述

  • 产品名称人LIGHT ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.25 86% - 105%
    Serum 95.58 84% - 103%
    Plasma 97.63 85% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s LIGHT Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIGHT in serum, plasma, and cell culture supernatants. (Human LIGHT concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human LIGHT coated on a 96-well plate. Standards and samples are pipetted into the wells and LIGHT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIGHT antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIGHT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
  • 组织特异性Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰N-glycosylated.
    The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
  • 细胞定位Cytoplasm; Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • CD258
    • Delta transmembrane LIGHT
    • Herpes virus entry mediator ligand
    • Herpesvirus entry mediator A
    • Herpesvirus entry mediator ligand
    • HVEM L
    • HVEM-L
    • HVEML
    • Ligand for herpesvirus entry mediator
    • LTg
    • soluble form
    • TNF14_HUMAN
    • Tnfsf14
    • Tumor necrosis factor (ligand) superfamily member 14
    • Tumor necrosis factor ligand superfamily member 14
    • Tumor necrosis factor receptor like 2
    • Tumor necrosis factor superfamily member LIGHT
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human LIGHT ELISA Kit 图像

  • Representative Standard Curve using ab100583.

  • Representative Standard Curve using ab100583.

实验方案

Human LIGHT ELISA Kit (ab100583)参考文献

ab100583 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I can confirm that the ab100583 LIGHT Human ELISA Kit is QC tested with EDTA, heparin and citrate anti-coagulants so all 3 will be compatible.  Other anticoagulants have not been tested. I hope this will be helpful t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"