人Leptin ELISA试剂盒(ab108879)

概述

  • 产品名称
    人Leptin ELISA试剂盒
    参阅全部 Leptin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.7%
    批次间
    样品 n Mean SD CV%
    Overall 7%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.12 ng/ml
  • 范围
    0.125 ng/ml - 32 ng/ml
  • 复检

    = 99 %

  • 检测时间
    5h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Leptin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin concentrations in Human cell culture supernatants, serum and plasma.


    A Leptin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Leptin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leptin captured in plate.


    Get results in 90 minutes with Human Leptin ELISA Kit (ab179884) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab108879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108879.

实验方案

文献

ab108879 has not yet been referenced specifically in any publications.

客户评价及客户问答

These products are very similar, they use similar techniques to measure human Leptin in samples. In reviewing the information about each kit I have noted that ab100581 Sensitivity< 2 pg/ml, Range of 1.64 pg/ml - 400 pg/ml and Recovery of 94%. While ...

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Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laborat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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