人Anti-Leishmania IgG ELISA试剂盒(ab108749)

概述

  • 产品名称人Anti-Leishmania IgG ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Pos.Serum 7 7.4%
    批次间
    样品 n Mean SD CV%
    Pos.Serum 4 5.9%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Indirect
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Leishmania IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Leishmania in Human serum and plasma.

     

    A 96-well plate has been precoated with Leishmania antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Leishmania-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leishmania IgG sample captured in plate.

  • 经测试应用适用于: Indirect ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover foil 1 unit
    IgG Sample Diluent Yellow, White cap 1 x 100ml
    Leishmania (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 1 unit
    Leishmania IgG Cut-off Control Yellow, Green cap 1 x 3ml
    Leishmania IgG Negative Control Yellow, Blue cap 1 x 2ml
    Leishmania IgG Positive Control Yellow, Red cap 1 x 2ml
    Leishmania Protein A Conjugate Blue, Black cap 1 x 20ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab108749 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Indirect ELISA Use at an assay dependent dilution.

    实验方案

    Human Anti-Leishmania IgG ELISA Kit (ab108749)参考文献

    ab108749 has not yet been referenced specifically in any publications.

    Product Wall

    This product has been tested with L.infantum in human serum or plasma collected with citrate. Other Leishmania species hasn’t been tested so we are unsure how well this kit will perform. In case you are interested in buying and trying this produc...

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