人LEC ELISA试剂盒(CCL16) (ab100532)

概述

  • 产品名称
    人LEC ELISA试剂盒(CCL16)
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.47 85% - 104%
    Serum 95.29 84% - 103%
    Plasma 94.68 83% - 103%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s LEC (CCL16) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LEC in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human LEC coated on a 96-well plate. Standards and samples are pipetted into the wells and LEC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LEC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LEC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human LEC 2 vials
    LEC Microplate (12 x 8 wells) 1 unit
    Recombinant Human LEC Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
  • 组织特异性
    Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
  • 序列相似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • C-C motif chemokine 16
    • CCL16
    • CCL16_HUMAN
    • Chemokine CC-4
    • Chemokine LEC
    • CKb12
    • HCC-4
    • HCC4
    • IL-10-inducible chemokine
    • IL10 inducible chemokine
    • ILINCK
    • LCC-1
    • LCC1
    • Liver CC chemokine 1 precursor
    • Liver expressed chemokine
    • Liver-expressed chemokine
    • LMC
    • Lymphocyte and monocyte chemoattractant
    • Monotactin 1
    • Monotactin-1
    • MTN-1
    • Mtn1
    • NCC-4
    • NCC4
    • New CC chemokine 4
    • SCYA16
    • SCYL4
    • Small inducible cytokine A16 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 16
    • Small-inducible cytokine A16
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100532

  • Representative standard curve using ab100532

实验方案

文献

ab100532 has not yet been referenced specifically in any publications.

客户评价及客户问答

LEC concentration in 16 human serum samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human sera were diluted 10 times by Diluent A.
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提交于 Jun 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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