The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Ku70 antibody (ab83501)
纯度70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
5''-deoxyribose-5-phosphate lyase Ku70
5''-dRP lyase Ku70
70 kDa subunit of Ku antigen
ATP dependent DNA helicase 2 subunit 1
ATP dependent DNA helicase II 70 kDa subunit
ATP-dependent DNA helicase 2 subunit 1
ATP-dependent DNA helicase II 70 kDa subunit
CTC box binding factor 75 kDa subunit
CTC box-binding factor 75 kDa subunit
DNA repair protein XRCC6
Ku autoantigen p70 subunit
Ku autoantigen, 70kDa
Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa
Lupus Ku autoantigen protein p70
Thyroid autoantigen 70kD (Ku antigen)
Thyroid lupus autoantigen
Thyroid lupus autoantigen p70
X ray repair complementing defective repair in Chinese hamster cells 6
X-ray repair complementing defective repair in Chinese hamster cells 6
X-ray repair cross-complementing protein 6
功能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
序列相似性Belongs to the ku70 family. Contains 1 Ku domain. Contains 1 SAP domain.
发展阶段Expression does not increase during promyelocyte differentiation.
翻译后修饰Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.