人Kininogen ELISA试剂盒(KNG) (ab108876)
Key features and details
- Sensitivity: 0.7 ng/ml
- Range: 1.563 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Milk, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Kininogen ELISA试剂盒(KNG)
参阅全部 Kininogen 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.5% 批次间 样品 n Mean SD CV% Overall 9.1% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.7 ng/ml -
范围
1.563 ng/ml - 100 ng/ml -
回收率
96 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Kininogen (KNG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kininogen in urine, saliva, milk, cerebrospinal fluid and cell culture supernatants.
A Kininogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Kininogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Kininogen captured in plate.
Get results in 90 minutes with Human HMW Kininogen ELISA Kit (ab189574) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Kininogen Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Kininogen Microplate (12 x 8 well strips) 1 unit Kininogen Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究领域
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功能
(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. -
组织特异性
Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors. -
疾病相关
Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. -
序列相似性
Contains 3 cystatin domains. -
翻译后修饰
Bradykinin is released from kininogen by plasma kallikrein.
Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
Phosphorylation sites are present in the extracelllular medium.
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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别名
- Alpha-2-thiol proteinase inhibitor
- BDK
- BK
see all -
数据库链接
- Entrez Gene: 3827 Human
- Omim: 612358 Human
- SwissProt: P01042 Human
- Unigene: 77741 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108876 被引用在 3 文献中.
- Markaki I et al. Cerebrospinal Fluid Levels of Kininogen-1 Indicate Early Cognitive Impairment in Parkinson's Disease. Mov Disord 35:2101-2106 (2020). PubMed: 33179332
- Zou J et al. Kininogen Level in the Cerebrospinal Fluid May Be a Potential Biomarker for Predicting Epileptogenesis. Front Neurol 10:37 (2019). PubMed: 30804871
- Niu R et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . PubMed: 28122020