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Abcam’s Kininogen (KNG) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kininogen in plasma and serum samples.
A Kininogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Kininogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Kininogen captured in plate.
Get results in 90 minutes with Human HMW Kininogen ELISA Kit (ab189574) from our SimpleStep ELISA® range.
|组件||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|1X Biotinylated Human Kininogen (Lyophilized)||1 vial|
|20X Wash Buffer Concentrate||1 x 30ml|
|Chromogen Substrate||1 x 8ml|
|Kininogen Microplate (12 x 8 well strips)||1 unit|
|Kininogen Standard||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
Kininogen measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 400-800 fold.
Representative Standard Curve using ab108875.
ab108875 has not yet been referenced specifically in any publications.