人Kallikrein 1 ELISA试剂盒(KLK1) (ab119598)

概述

  • 产品名称人Kallikrein 1 ELISA试剂盒(KLK1)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Homogenate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    156 pg/ml - 10000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human KLK1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human KLK1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

    A KLK1 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KLK1 is then added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human KLK1 amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • 组织特异性Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • 序列相似性Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
  • Information by UniProt
  • 别名
    • Glandular kallikrein 1
    • hK1
    • Kallikrein 1 renal/pancreas/salivary
    • Kallikrein serine protease 1
    • Kallikrein-1
    • Kidney/pancreas/salivary gland kallikrein
    • KLK1
    • KLK1_HUMAN
    • Klk6
    • KLKR
    • Tissue kallikrein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Kallikrein 1 ELISA Kit (KLK1) 图像

  • Representative Standard Curve using ab119598

实验方案

Human Kallikrein 1 ELISA Kit (KLK1) (ab119598)参考文献

ab119598 has not yet been referenced specifically in any publications.

Product Wall

Detection of human KLK1 in saliva is likely but unconfirmed by experiment. The antibodies in the assay should be capable of recognizing both isoforms 1 and 2, which are described at the UniProt entry at this link. http://www.uniprot.org/uniprot/P06870....

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This product will work for cell culture supernatants. Prepare samples as directed below: Cell culture supernates or tissue lysates: Remove particulates by centrifugation, assay immediately or aliquot and store samples at -20°C.

Thank you for your inquiry.

We have never tested this ELISA kit with urine samples (only serum, plasma, and tissue extracts) so we do not know if it will work. Theoretically if urine has KLK1 concentrations within the range of detection 156 p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"