人IP-10 ELISA试剂盒(ab100579)

概述

  • 产品名称人IP-10 ELISA试剂盒
    参阅全部 IP10 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 101.34 84% - 105%
    Serum 94.49 82% - 104%
    Plasma 96.13 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IP-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IP-10 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IP-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IP-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IP-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Interferon gamma induced factor MOB1, mouse, homolog of
    • Interferon gamma induced protein 10
    • 10 kDa interferon gamma induced protein
    • 10 kDa interferon gamma-induced protein
    • C X C motif chemokine 10
    • C7
    • Chemokine (C X C motif) ligand 10
    • Chemokine CXC motif ligand 10
    • Crg 2
    • CRG2
    • CXCL10
    • CXCL10(1-73)
    • CXL10_HUMAN
    • Gamma IP10
    • Gamma-IP10
    • gIP 10
    • GIP10
    • IFI10
    • INP 10
    • INP10
    • Interferon activated gene 10
    • Interferon gamma induced cell line
    • Interferon inducible cytokine IP 10
    • Interferon inducible cytokine IP10
    • IP 10
    • IP-10
    • Mob 1
    • MOB1
    • Protein 10 from interferon (gamma) induced cell line
    • SCYB10
    • Small inducible cytokine B10
    • Small inducible cytokine B10 precursor
    • Small inducible cytokine subfamily B (Cys X Cys) member 10
    • Small inducible cytokine subfamily B CXC member 10
    • Small inducible cytokine subfamily B, member 10
    • Small-inducible cytokine B10
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IP-10 ELISA Kit 图像

  • Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).

  • Standard curve in dilutent A with background signal subtracted (duplicates; +/- SD).

  • IP-10 measured in undiluted cell culture supernatants from control (-) or treated ((PHA) 2% PHA-M; LifeTechnologies) PBMCs or in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (P/L) (duplicates +/- SD).

  • IP-10 measured in undiluted biological fluids (duplicates +/- SD). Signals from mouse and rat samples were below level of detection.

  • Representative Standard Curve using ab100578.

  • Representative Standard Curve using ab100578.

实验方案

Human IP-10 ELISA Kit (ab100579)参考文献

ab100579 has not yet been referenced specifically in any publications.

Product Wall

IP-10 Human ELISA

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Easy to use and easy to follow ELISA kit. Would recommend this kit for cell culture samples.
Username

Abcam user community

Verified customer

提交于 Jul 21 2015

CXCL10 secretion by macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CXCL10 secretion my human primary macrophages in culture for 3 days.
Human monocytes isolated by negative selection.
CXCL10 secretion by the M1 polarized macrophages is observed.
The kit worked perfectly well, no dilution necessary, the assay was performed in 5.30hr.
Username

Abcam user community

Verified customer

提交于 Mar 12 2014

Thank you for contacting us.

We unfortunately do not have these kits available for human samples. I however have contacted the product manager and asked them if these products are in pipeline, I will get back to you once I get it confirmed. Read More

I have contacted the lab who confirmed that it is not recommended to load less than 100 ul of sample per well as this may affect the quality of the results. For the best results, the protocol should be followed exactly.

Thank you for your inquiry. Unfortunately, there is no cross reactivity data available testing cynomolgus with the Human IP-10 ELISA kit (ab100579). However, I would expect the homology between cynomolgus and human to be quite high so I think it wo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"