Anti-Human intestinal alkaline phosphatase抗体(ab97532)

概述

  • 产品名称Anti-Human intestinal alkaline phosphatase抗体
    参阅全部 intestinal alkaline phosphatase 一抗
  • 描述
    兔多克隆抗体to人intestinal alkaline phosphatase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 3 and 216 of Human intestinal alkaline phosphatase (NP_001622)

  • 阳性对照
    • 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysate. HepG2 whole cell lysate. Paraffin-embedded SG xenograft

性能

应用

Our Abpromise guarantee covers the use of ab97532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500.

靶标

Anti-Human intestinal alkaline phosphatase antibody 图像

  • Anti-Human intestinal alkaline phosphatase antibody (ab97532) at 1/1000 dilution + Hep G2 whole cell lsyate at 30 µg

    Predicted band size : 57 kDa
  • Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab97532 at 1/100 dilution.

Anti-Human intestinal alkaline phosphatase antibody (ab97532)参考文献

This product has been referenced in:
  • Shin J  et al. The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner. J Biol Chem 289:25306-16 (2014). WB ; Human . Read more (PubMed: 25037223) »

See 1 Publication for this product

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Thank you for submitting an Abreview of ab97532. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species (rat), we would like to follow up on this....

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (small intestine)
Loading amount 25 µg
Specification small intestine
Gel Running Conditions Reduced Denaturing (8 % gel)
Blocking step 3% BSA, 5% Milk, Blocking Buffer for fluorescent Western Blotting as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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Verified customer

提交于 May 12 2012

DISCOUNT CODE: xxx Ablaufdatum: dd.mm.2012 Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab97532 in der Ratte zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetze...

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Vielen Dank für Ihre Anfrage. Das Immunogen, das zur Herstellung von ab95462 verwendet wird, ist ein synthetisches Peptid, das von der Alkalischen Phosphatase der Ratte mit der SwissProt-ID P08289 abgeleitet ist. Leider weist dieses Peptid keine Seq...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"