人Interferon Gamma + Perforin酶联免疫斑点法(ELISPOT)试剂盒(without plates) (ab59703)

概述

  • 产品名称人Interferon Gamma + Perforin酶联免疫斑点法(ELISPOT)试剂盒(without plates)
    参阅全部 Interferon Gamma + Perforin 试剂盒
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型Sandwich (qualitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates. The dual colour Elispot allows you to monitor the production of two cytokines simultaneously in the same well.

     

    Recognizes natural human Interferon gamma and Perforin

  • 平台Reagents

性能

Human Interferon Gamma + Perforin ELISPOT Kit (without plates) (ab59703)参考文献

ab59703 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59703.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"