人Interferon Gamma + IL2 FLUOROSPOT试剂盒(without plates) (ab48451)

概述

  • 产品名称人Interferon Gamma + IL2 FLUOROSPOT试剂盒(without plates)
    参阅全部 Interferon Gamma + IL2 试剂盒
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型Sandwich (qualitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

     

    Principle

    After cell stimulation, locally produced cytokines are captured by IFN gamma and IL2 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- IFN gamma FITC conjugated antibody and a biotinylated anti-IL2 antibody. Those are in turn recognised by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IFN gamma production while IL2 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.

  • 经测试应用适用于: FluroSpotmore details
  • 平台Reagents

性能

  • 相关性IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 细胞定位Secreted
  • 别名
    • IFN gamma
    • IFNG
    • IL2
    • Immune interferon
    • Interferon gamma
    • Interleukin 2
    • Lymphokine
    • TCGF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab48451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FluroSpot Use at an assay dependent dilution.

Human Interferon Gamma + IL2 FLUOROSPOT Kit (without plates) (ab48451)参考文献

ab48451 has not yet been referenced specifically in any publications.

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1) Im IL2 ELISpot Kitab46546 sind nur die Reagenzien für den ELISpot enthalten, keine Platten. Den IL2 ELISpotKitMIT Platten haben wir alsab62927 erhältlich:

http://www.abcam.com/index.htm...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"