人Anti-Influenza virus B IgM ELISA试剂盒(ab108748)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
人Anti-Influenza virus B IgM ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos. Serum 8 5.9% 批次间 样品 n Mean SD CV% Pos. Serum 12 7.5% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Influenza virus B IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Influenza virus B in Human serum and plasma.
A 96-well plate has been precoated with Influenza virus B antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Influenza virus B-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus B IgM sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgM Sample Diluent Green, White cap 1 x 100ml Influenza virus B (IgM) Coated Microplate (12 x 8 wells) 1 unit Influenza virus B anti-IgM HRP Conjugate Red, Black cap 1 x 20ml Influenza Virus B IgM Cut off Control Yellow, Green cap 1 x 3ml Influenza Virus B IgM Negative Control Yellow, Blue cap 1 x 2ml Influenza Virus B IgM Positive Control Yellow, Red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
相关产品
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Related Products
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108748 被引用在 1 文献中.
- James S et al. Adults with Trisomy 21 Have Differential Antibody Responses to Influenza A. Vaccines (Basel) 10:N/A (2022). PubMed: 35891309