人IL8酶联免疫吸附(ELISA)套装(without plates) (ab48481)

概述

  • 产品名称
    人IL8酶联免疫吸附(ELISA)套装(without plates)
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    2000 pg/ml - 62.5 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-8 Human in vitro ELISA Set is designed for the quantitative measurement of IL-8 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


    The IL8 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


    This ELISA set is intended to measure human IL8 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Sensitivity: < 29 pg / ml

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Reagents

性能

应用

Our Abpromise guarantee covers the use of ab48481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA
  • 应用说明
    ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 图片

    • IL-8 Standard curve ranging from 62.5 to 2000 pg/mL

    文献

    This product has been referenced in:
    • Liang CL  et al. Protection Effect of Zhen-Wu-Tang on Adriamycin-Induced Nephrotic Syndrome via Inhibiting Oxidative Lesions and Inflammation Damage. Evid Based Complement Alternat Med 2014:131604 (2014). ELISA . Read more (PubMed: 24812565) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for contacting us. Overnight Incubation of plate at 4C is sort of general recommendation we provide to our customers. 3-4 hours at 4C can also be tried; the assay should be OK However in case of no readings we would be suggesting the over...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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