人IL7酶联免疫吸附(ELISA)套装(without plates) (ab47357)

概述

  • 产品名称
    人IL7酶联免疫吸附(ELISA)套装(without plates)
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    6.25 pg/ml - 200 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-7 Human in vitro ELISA Set is designed for the quantitative measurement of IL-7 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


    This ELISA Set is intended to measure human IL-7 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    These reagents are intended for research use only, not for diagnostic purposes.


    The IL-7 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


    Recognizes both natural and recombinant human IL7.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Reagents

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-7 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-7 Capture antibody 1 x 0.25ml 2 x 0.25ml 3 x 0.25ml
    IL7 standard Yellow 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • 研究领域
  • 相关性
    Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
  • 细胞定位
    Secreted
  • 别名
    • IL 7
    • Interleukin 7
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab47357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • IL-7 Standard curve ranging from 6.25 to 200 pg/mL

文献

ab47357 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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