人IL-7 ELISA试剂盒(without plates) (ab47357)
概述
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产品名称
人IL-7 ELISA试剂盒(without plates)
参阅全部 IL7 试剂盒 -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 3 pg/ml -
范围
6.25 pg/ml - 200 pg/ml -
检测时间
2h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-7 Human in vitro ELISA Set is designed for the quantitative measurement of IL-7 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human IL-7 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
These reagents are intended for research use only, not for diagnostic purposes.
The IL-7 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL-7.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 15 x 96 tests 5 x 96 tests 10 x 96 tests Chromogen TMB Substrate Solution 6 vials 2 vials 4 vials Detection biotinylated anti-IL-7 antibody 3 x 0.55ml 1 x 0.55ml 2 x 0.55ml IL-7 Capture antibody 3 x 0.25ml 1 x 0.25ml 2 x 0.25ml IL-7 standard Yellow 15 vials 5 vials 10 vials Streptavidin-HRP 3 vials 1 vial 2 vials -
研究领域
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相关性
Interleukin 7 (IL-7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. -
细胞定位
Secreted -
别名
- IL 7
- IL7
- Interleukin 7
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数据库链接
- Entrez Gene: 3574 Human
- SwissProt: P13232 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47357于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
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