人IL5酶联免疫吸附(ELISA)套装(without plates) (ab47356)

概述

  • 产品名称
    人IL5酶联免疫吸附(ELISA)套装(without plates)
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    7.8 pg/ml - 250 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-5 Human in vitro ELISA Set is designed for the quantitative measurement of IL-5 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human IL-5 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    These reagents are intended for research use only, not for diagnostic purposes.

     

    The IL-5 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human IL5.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Reagents

性能

  • 相关性
    Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology.
  • 细胞定位
    Secreted
  • 别名
    • B cell differentiation factor I
    • Eosinophil differentiation factor
    • Interleukin 5
    • T cell replacing factor
    • TRF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab47356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL5 ELISA Set (without plates) 图像

  • IL-5 Standard curve ranging from 7.8 to 250 pg/mL

Human IL5 ELISA Set (without plates) (ab47356)参考文献

ab47356 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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