人IL4酶联免疫斑点法(ELISPOT)试剂盒(ab46557)

概述

  • 产品名称
    人IL4酶联免疫斑点法(ELISPOT)试剂盒
    参阅全部 IL4 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型
    Sandwich (qualitative)
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Intended Use

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.


    Principle of Method

    Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

     

    Recognizes natural human IL4

  • 经测试应用
    适用于: ELISpotmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 5 x 96 tests 10 x 96 tests
    96 PVDF-bottomed-well plates. 5 units 10 units
    Bovine Serum Albumin 1 x 1g 2 x 1g
    IL-4 Biotinylated detection antibody 1 vial 2 vials
    IL-4 Capture antibody 1 x 500µl 2 x 500µl
    Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl
  • 研究领域
  • 相关性
    IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
  • 细胞定位
    Secreted
  • 别名
    • B cell stimulatory factor 1
    • Binetrakin
    • BSF1
    • Interleukin 4
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISpot
  • 应用说明



  • 文献

    ab46557 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab46557.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册