人IL2 Receptor alpha ELISA试剂盒(without plates) (ab47354)

概述

  • 产品名称
    人IL2 Receptor alpha ELISA试剂盒(without plates)
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    68.75 pg/ml - 2220 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-2 Receptor (IL-2R) Human in vitro ELISA Set is designed for the quantitative measurement of IL-2R in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human IL-2R in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    The IL-2R ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


    Recognizes both natural and recombinant human IL2R

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Reagents

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-2R antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-2R Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    Streptavidin-HRP 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • 研究领域
  • 功能
    Receptor for interleukin-2.
  • 疾病相关
    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • 序列相似性
    Contains 2 Sushi (CCP/SCR) domains.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • CD25
    • IL-2 receptor subunit alpha
    • IL-2-RA
    • IL-2R subunit alpha
    • IL2 receptor alpha subunit
    • IL2-RA
    • IL2R
    • IL2RA
    • IL2RA_HUMAN
    • Interleukin-2 receptor subunit alpha
    • p55
    • TAC antigen
    • TCGFR
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab47354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • sIL-2R Standard curve ranging from 68.75 to 2200 pg/mL

文献

ab47354 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. The two soluble IL2 receptor ELISA kits, ab46036 (with plate) and ab47354 (capture and detection antibodies sufficient for five plates, but plates are not included) are known to be reactive with the alpha sub-unit. Reactivi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册