概述

  • 产品名称
    人IL1RA ELISA试剂盒
    参阅全部 IL1RA 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 0.1 ng/ml
  • 范围
    0.124 ng/ml - 30 ng/ml
  • 复检

    92 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 93.45 93% - 103%
    Tissue Extracts 91.92 82% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human IL1RA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL1RA in cell lysates and tissue lysates.


    This assay employs an antibody specific for Human IL1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2x Cell lysate buffer 1 x 5ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-Human IL-1RA 2 vials
    IL-1RA Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-1RA Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • 组织特异性
    The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • 疾病相关
    Microvascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • 细胞定位
    Cytoplasm and Secreted.
  • Information by UniProt
  • 别名
    • DIRA
    • F630041P17Rik
    • ICIL 1RA
    • ICIL-1RA
    • ICIL1RA
    • IL-1ra
    • IL-1ra3
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RA_HUMAN
    • IL1RN
    • IL1RN (IL1F3)
    • Interleukin 1 receptor antagonist
    • Interleukin-1 receptor antagonist protein
    • Intracellular IL 1 receptor antagonist type II
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra)
    • IRAP
    • MGC10430
    • MVCD4
    • Type II interleukin 1 receptor antagonist
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • IL1RA measured in RIPA lysates from control cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours (IL-1Ra per million cells; duplicates +/- SD). 5e6 cells were lysed in 0.1 mL, and tested in the dilution range of 1/1-1/40.  

  • IL1RA measured in culture supernatants from control (-) or activated PBMCs ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and in human biological fluids. Signals from plasma and serum were below level of detection (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100565

实验方案

文献

This product has been referenced in:
  • Leaw B  et al. Human amnion epithelial cells rescue cell death via immunomodulation of microglia in a mouse model of perinatal brain injury. Stem Cell Res Ther 8:46 (2017). Read more (PubMed: 28241859) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us.
Thank you for your inquiry. Unfortunately we have not tested this kit for cross reactivity with rat. If it helps, the capture antibody used is monoclonal and the detection antibody is polyclonal. If you do wish to test...

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