概述

  • 产品名称人IL1RA ELISA试剂盒
    参阅全部 IL1RA 试剂盒
  • 检测方法Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.1 ng/ml
  • 范围
    0.124 ng/ml - 30 ng/ml
  • 复检

    99 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 99.35 89% - 107%
    Plasma 100.27 90% - 108%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL1RA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL1RA in plasma and cell culture supernatants. (Human IL1RA concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human IL1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL1RA in normal serum with ab100564, only in serum spiked with Human IL1RA.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • 组织特异性The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • 疾病相关Microvascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • 细胞定位Cytoplasm and Secreted.
  • Information by UniProt
  • 别名
    • DIRA
    • F630041P17Rik
    • ICIL 1RA
    • ICIL-1RA
    • ICIL1RA
    • IL-1ra
    • IL-1ra3
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RA_HUMAN
    • IL1RN
    • IL1RN (IL1F3)
    • Interleukin 1 receptor antagonist
    • Interleukin-1 receptor antagonist protein
    • Intracellular IL 1 receptor antagonist type II
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra)
    • IRAP
    • MGC10430
    • MVCD4
    • Type II interleukin 1 receptor antagonist
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL1RA ELISA Kit 图像

  • Representative standard curve using ab100564

  • Representative standard curve using ab100564

实验方案

Human IL1RA ELISA Kit (ab100564)参考文献

ab100564 has not yet been referenced specifically in any publications.

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