人IL17酶联免疫吸附(ELISA)套装(without plates) (ab83707)

概述

  • 产品名称人IL17酶联免疫吸附(ELISA)套装(without plates)
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    3.125 pg/ml - 100 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-17 Human in vitro ELISA Set is designed for the quantitative measurement of IL-17 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL-17A ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure human IL-17A in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    SPECIFICITY: Recognizes both natural and recombinant human IL-17A

    RANGE: 3.12pg/ml - 100pg/ml

    SENSITIVITY: <2.3pg/ml

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Reagents

性能

  • 相关性IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts; the enhancement of surface expression of ICAM 1 in fibroblasts; activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5% amino acid homology to mouse IL17 and 58% amino acid homology to rat IL17.
  • 细胞定位Secreted
  • 别名
    • CTLA 8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • IL 17A
    • IL17A
    • Interleukin 17A
    • Interleukin17
    • Interleukin17A
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab83707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL17 ELISA Set (without plates) 图像

  • IL-17 Standard curve ranging from 3.125 to 100 pg/mL

Human IL17 ELISA Set (without plates) (ab83707)参考文献

ab83707 has not yet been referenced specifically in any publications.

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