人IL10 + IL2 FLUOROSPOT试剂盒(ab48460)

概述

  • 产品名称人IL10 + IL2 FLUOROSPOT试剂盒
    参阅全部 IL10 + IL2 试剂盒
  • 检测方法Fluorescent
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型Sandwich (qualitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principle of Method

    After cell stimulation, locally produced cytokines are captured by IL-10 and IL-2 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- IL-10 FITC conjugated antibody and a biotinylated anti-IL-2 antibody. Those are in turn recognised by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IL-10 production while IL-2 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.

  • 经测试应用适用于: FluroSpotmore details
  • 平台Microplate

性能

  • 相关性IL2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. IL10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 细胞定位Secreted
  • 别名
    • Aldesleukin
    • CSIF
    • Cytokine synthesis inhibitory factor
    • Interleukin 10
    • Interleukin 2
    • Lymphokine
    • T cell growth factor
    • TCGF
    • TGIF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab48460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
FluroSpot Use at an assay dependent dilution.

Human IL10 + IL2 FLUOROSPOT Kit (ab48460)参考文献

ab48460 has not yet been referenced specifically in any publications.

Product Wall

Für ist eine Detektion diese Fluorospot Kits ist UV Licht zwingend notwendig. Dies kann mit einem UV-Licht Mikroskop für die Auszählung der Punkte per Hand geschehen oder auch mit einem ELISPOT Reader, der eine UV Lampe besitzt (z.B. von CTL, Zeiss ode...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"