人IL-10 + IL-2 FLUOROSPOT Set (ab48460)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Fluorescent
- Sample type: Suspension cells
- Reacts with: Human
概述
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产品名称
人IL-10 + IL-2 FLUOROSPOT Set -
检测方法
Fluorescent -
样品类型
Suspension cells -
检测类型
Sandwich (qualitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principle of Method
After cell stimulation, locally produced cytokines are captured by IL-10 and IL-2 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- IL-10 FITC conjugated antibody and a biotinylated anti-IL-2 antibody. Those are in turn recognised by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IL-10 production while IL-2 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.
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经测试应用
适用于: FluroSpotmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 5 x 96 tests 10 x 96 tests 96 PVDF-bottomed-well plates. 5 units 10 units Anti-FITC antibody green fluorescence conjugate. 1 vial 2 vials Biotinylated detection antibody for IL-2 (resuspend in 0.55mL). 1 vial 2 vials Bovine Serum Albumin 1 x 1g 2 x 1g Human IL-10 Capture antibody 1 x 500µl 2 x 500µl Capture antibody for IL-2 1 x 500µl 2 x 500µl FITC conjugated detection antibody for IL-10(resuspend in 0.55mL). 1 vial 2 vials Streptavidin-phycoerythrin conjugate. 1 vial 2 vials -
研究领域
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相关性
IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
细胞定位
Secreted -
别名
- Aldesleukin
- CSIF
- Cytokine synthesis inhibitory factor
see all -
数据库链接
- Entrez Gene: 3558 Human
- Entrez Gene: 3586 Human
- SwissProt: P60568 Human
- SwissProt: P22301 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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FluroSpot |
Use at an assay dependent dilution.
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说明 |
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FluroSpot
Use at an assay dependent dilution. |
数据表及文件
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SDS download
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Datasheet download
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