人IL10 + IL2酶联免疫斑点法(ELISPOT)试剂盒(without plates) (ab48455)

概述

  • 产品名称人IL10 + IL2酶联免疫斑点法(ELISPOT)试剂盒(without plates)
    参阅全部 IL10 + IL2 试剂盒
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型Sandwich (qualitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    The dual colour Elispot allows you to monitor the production of two cytokines simultaneously in the same well.


    Principle

     

    After cell stimulation, locally produced cytokines are captured by IL10 and IL2 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti-IL10 FITC conjugated antibody and a biotinylated anti-IL2 antibody. Those are in turn recognised by anti-FITC HRP and streptavidin-AP conjugates. PVDF-bottomed-well plates are then incubated first with AEC substrate buffer, washed and subsequently incubated with BCIP/NBT. Coloured red/brownish spots indicate IL10 production while IL2 is revealed by blue/purple spots.

  • 经测试应用适用于: ELISpotmore details
  • 平台Reagents

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests
    10 x concentrate buffer for the preparation of AEC buffer 1 x 5ml 2 x 5ml 3 x 5ml 4 x 5ml
    50 x concentrate AEC substrate buffer 1 x 1ml 2 x 1ml 3 x 1ml 4 x 1ml
    Anti-FITC antibody HRP conjugate 1 x 100µl 2 x 100µl 3 x 100µl 4 x 100µl
    Bovine Serum albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g
    Human IL-10 Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl
    Human IL-2 Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl
    IL10 FITC conjugated detection antibody 1 vial 2 vials 3 vials 4 vials
    IL-2 Biotinylated detection antibody 1 vial 2 vials 3 vials 4 vials
    Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 4 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl
  • 研究领域
  • 相关性IL2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. IL10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 细胞定位Secreted
  • 别名
    • Aldesleukin
    • CSIF
    • Cytokine synthesis inhibitory factor
    • Interleukin 10
    • Interleukin 2
    • Lymphokine
    • T cell growth factor
    • TCGF
    • TGIF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab48455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISpot Use at an assay dependent dilution.

Human IL10 + IL2 ELISPOT Kit (without plates) (ab48455)参考文献

ab48455 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"