人IL10酶联免疫斑点法(ELISPOT)试剂盒(试剂for 10 x 96 Well Plates) (ab46601)

概述

  • 产品名称人IL10酶联免疫斑点法(ELISPOT)试剂盒(试剂for 10 x 96 Well Plates)
    参阅全部 IL10 试剂盒
  • 测试
    10 x 96 well plate
  • 检测类型Sandwich (quantitative)
  • 种属反应性
    与反应: Human
  • 产品概述

    Intended use

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    The Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

     

    Recognizes natural human IL10

  • 经测试应用适用于: ELISpotmore details

性能

  • 功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 组织特异性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 序列相似性Belongs to the IL-10 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • IL-10
    • IL10
    • IL10_HUMAN
    • Interleukin 10
    • Interleukin-10
    • TGIF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISpot
  • 应用说明ELISPOT
  • Human IL10 ELISPOT Kit (Reagents for 10 x 96 Well Plates) (ab46601)参考文献

    ab46601 has not yet been referenced specifically in any publications.

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