人IL10酶联免疫吸附(ELISA)套装(without plates) (ab48485)

概述

  • 产品名称人IL10酶联免疫吸附(ELISA)套装(without plates)
    参阅全部 IL10 试剂盒
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    400 pg/ml - 12.5 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-10 Human in vitro ELISA Set is designed for the quantitative measurement of IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure human IL10 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Sensitivity: < 5 pg / ml

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Reagents

性能

  • 功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 组织特异性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 序列相似性Belongs to the IL-10 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • IL-10
    • IL10
    • IL10_HUMAN
    • Interleukin 10
    • Interleukin-10
    • TGIF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab48485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Human IL10 ELISA Set (without plates) 图像

    • IL-10 Standard curve ranging from 12.5 to 400 pg/mL

    Human IL10 ELISA Set (without plates) (ab48485)参考文献

    ab48485 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"