人IL1 beta酶联免疫吸附(ELISA)套装(without plates) (ab47351)

概述

  • 产品名称人IL1 beta酶联免疫吸附(ELISA)套装(without plates)
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    15.6 pg/ml - 500 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-1β Human in vitro ELISA Set is designed for the quantitative measurement of IL-1β in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human IL-1b in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    The IL-1b ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human IL1b.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Reagents

性能

  • 相关性Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
  • 细胞定位Secreted; the lack of a specific hydrophobic segment in the precursor sequence suggests that IL1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • 别名
    • Catabolin
    • IL1 BETA
    • IL1B
    • IL1F2
    • interleukin 1, beta
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab47351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Human IL1 beta ELISA Set (without plates) 图像

    • IL1 beta Standard curve ranging from 15.6 to 500 pg/ml

    Human IL1 beta ELISA Set (without plates) (ab47351)参考文献

    ab47351 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam.



    The antibodies of ab47351 are both monoclonals, raised in mice.



    If there is anything else I can help you with, please let me know.

    Thank you for contacting us.

    1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) an...

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    Thank you for your enquiry.

    For ab47351 you can use 96 well ELISA plates. I can recommend to purchase ELISA plates specifically. to ensure they are of the higher quality required. I am sorry wedo not sell these from Abcam, I can recommendto ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"