人IL-1 alpha ELISA试剂盒(without plates) (ab47350)
概述
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产品名称
人IL-1 alpha ELISA试剂盒(without plates) -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-1α Human in vitro ELISA Set is designed for the quantitative measurement of IL-1α in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA set is intended to measure human IL-1 alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The IL-1 alpha ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL-1a.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 5 x 96 tests 10 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Detection biotinylated anti-IL-1 alpha antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml IL-1 alpha Capture antibody 1 x 0.1ml 2 x 0.1ml 3 x 0.1ml IL-1 alpha Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
研究领域
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相关性
IL-1 is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. -
别名
- Hematopoietin 1
- IL 1 alpha
- IL1 alpha
see all -
数据库链接
- Entrez Gene: 3552 Human
- SwissProt: P01583 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47350于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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