概述

  • 产品名称
    人IL-8 ELISA试剂盒(Interleukin 8)
    参阅全部 IL8 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Pooled Data 3.1%
    批次间
    样品 n Mean SD CV%
    Sample 1 18 1682pg/ml 152 9%
    Sample 2 18 1004pg/ml 111 11%
    Sample 3 18 768pg/ml 69 8.9%
    Sample 4 18 1497pg/ml 149 9.9%
    Sample 5 18 880pg/ml 83 9.4%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    62.5 pg/ml - 2000 pg/ml
  • 复检

    88 %

  • 检测时间
    1h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-8 supernatants, buffered solutions, serum and plasma samples. (Genecards: CXCL8)


    A monoclonal antibody specific for IL-8 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-8 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-8 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-8 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-8.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-8 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-8 Control Silver 2 vials 4 vials
    IL-8 Microplate (12 x 8 well strips) 1 unit 2 units
    IL8 Standard Yellow 2 vials 4 vials
    Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 功能
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 序列相似性
    Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 3-10C
    • AMCF-I
    • B-ENAP
    • Beta-thromboglobulin-like protein
    • C X C motif chemokine 8
    • C-X-C motif chemokine 8
    • Chemokine (C X C motif) ligand 8
    • CXCL8
    • Emoctakin
    • GCP 1
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL 8
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Interleukin 8
    • K60
    • LECT
    • LUCT
    • Lymphocyte derived neutrophil activating factor
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46032

  • IL-8 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. The positive control is included (Pos.). Results shown after background signal was subtracted (duplicates +/- SD).

实验方案

文献

This product has been referenced in:
  • Zhao C  et al. Long-term bicycle riding ameliorates the depression of the patients undergoing hemodialysis by affecting the levels of interleukin-6 and interleukin-18. Neuropsychiatr Dis Treat 13:91-100 (2017). ELISA ; Human . Read more (PubMed: 28096677) »
  • Prieto P  et al. Cell Expansion-Dependent Inflammatory and Metabolic Profile of Human Bone Marrow Mesenchymal Stem Cells. Front Physiol 7:548 (2016). ELISA ; Human . Read more (PubMed: 27899899) »

See all 10 Publications for this product

客户评价及客户问答

Nous ne recommandons pas de modifier les concentrations des courbes standards mais plutôt de diluer vos échantillons.

Toutefois, si vous voulez vraiment changer les courbes standards ceci est possible. Cependant en augmentant les ...

Read More

Thank you very much for your call yesterday and for your patience while I have been in touch with the lab about ab46032.

This kit is suitable for all body fluids. Urine samples should be diluted in standard diluent buffer (containing PBS BSA)...

Read More

Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high conc...

Read More

Thank you for your phone call yesterday and for your questions about our ELISA kits. I have heard back from the lab regarding ab46032, and unfortunately, the kit was never assayed on murine samples. So, I would not recommend this kit to use with mo...

Read More

Thank you for your enquiry. There's no Thimerosal in any component of the kit. However, we use azide (0.1%) in standard diluent and Biotinylated antibody diluent, and Proclin (0.1%) in HRP diluent.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册