人IL-8 ELISA试剂盒(Interleukin-8) (ab108869)

概述

  • 产品名称人IL-8 ELISA试剂盒(Interleukin-8)
    参阅全部 IL8 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.1%
    批次间
    样品 n Mean SD CV%
    Overall 7.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    > 0.01 ng/ml
  • 范围
    0.016 ng/ml - 1 ng/ml
  • 复检

    97 %

  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-8 (Interleukin-8) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-8 concentrations in Human cell culture supernatants, tissue extracts, serum and plasma.

    An IL-8 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-8 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-8 captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • Beta thromboglobulin like protein
    • C X C motif chemokine 8
    • C-X-C motif chemokine 8
    • Chemokine (C X C motif) ligand 8
    • CXCL8
    • Emoctakin
    • GCP 1
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL 8
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Interleukin 8
    • LECT
    • LUCT
    • Lymphocyte derived neutrophil activating factor
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-8 ELISA Kit (Interleukin-8) 图像

  • Representative Standard Curve using ab108869.

实验方案

Human IL-8 ELISA Kit (Interleukin-8) (ab108869)参考文献

ab108869 has not yet been referenced specifically in any publications.

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Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high conc...

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