人IL-7 ELISA试剂盒(Interleukin-7) (ab46031)

概述

  • 产品名称
    人IL-7 ELISA试剂盒(Interleukin-7)
    参阅全部 IL7 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 198.7pg/ml 1.667 0.83%
    Sample B 8 28.69pg/ml 1.118 3.86%
    批次间
    样品 n Mean SD CV%
    Sample A 29 198.9pg/ml 3.772 1.89%
    Sample B 29 25.68pg/ml 1.505 5.84%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    6 pg/ml - 200 pg/ml
  • 复检

    97.6 %

  • 检测时间
    2h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-7 in Human serum, plasma, buffered solutions and cell culture medium.


    A monoclonal antibody specific for IL-7 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-7 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-7 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-7 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-7.


    Get higher sensitivity in only 90 minutes with Human IL-7 ELISA Kit (ab185984) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-7 Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    IL-7 Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-7 Microplate (12 x 8 well strip) 1 unit 2 units
    IL7 standard Yellow 2 vials 4 vials
    Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性
    IL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • 细胞定位
    Secreted
  • 别名
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab46031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46031

实验方案

文献

ab46031 has not yet been referenced specifically in any publications.

客户评价及客户问答

Regrettably, the kit has not been validated with NIBSC IL-7 90/530. No calibration factor is available.

Sorry we could not be of further assistance in this instance. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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