人IL-7 ELISA试剂盒(Interleukin-7) (ab100574)

概述

  • 产品名称人IL-7 ELISA试剂盒(Interleukin-7)
    参阅全部 IL7 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    4.1 pg/ml - 1000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.41 83% - 106%
    Serum 95.63 84% - 106%
    Plasma 96.37 85% - 108%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-7 (Interleukin-7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-7 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性IL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • 细胞定位Secreted
  • 别名
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab100574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-7 ELISA Kit (Interleukin-7) 图像

  • Standard curve (Diluent A) with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100574

  • Human IL-7 measured in undiluted biological fluids (duplicates +/- SD).

  • Representative standard curve using ab100574

实验方案

Human IL-7 ELISA Kit (Interleukin-7) (ab100574)参考文献

ab100574 has not yet been referenced specifically in any publications.

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