人IL-6 Receptor ELISA试剂盒(Interleukin-6 Receptor) (ab46029)

概述

  • 产品名称人IL-6 Receptor ELISA试剂盒(Interleukin-6 Receptor)
    参阅全部 IL6R 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 7 977.7pg/ml 3.04 0.31%
    Sample B 7 131.1pg/ml 2.489 2.289%
    批次间
    样品 n Mean SD CV%
    Sample A 24 983.9pg/ml 10.46 1.06%
    Sample B 24 136.2pg/ml 6.086 4.46%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 复检

    96 %

  • 检测时间
    1h 40m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-6 Receptor (Interleukin-6 Receptor) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-6 Receptor in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-6 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 Receptor present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-6 Receptor.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • 组织特异性Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 结构域The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • 翻译后修饰A short soluble form may also be released from the membrane by proteolysis.
  • 细胞定位Secreted and Basolateral cell membrane.
  • Information by UniProt
  • 别名
    • CD126
    • CD126 antigen
    • Gp80
    • IL 6R alpha
    • IL-6 receptor subunit alpha
    • IL-6R 1
    • IL-6R subunit alpha
    • IL-6R-1
    • IL-6R-alpha
    • IL-6RA
    • Il6r
    • IL6R1
    • IL6RA
    • IL6RA_HUMAN
    • IL6Ralpha
    • Interleukin 6 receptor
    • Interleukin-6 receptor subunit alpha
    • Membrane glycoprotein 80
    • MGC104991
    • OTTHUMP00000034320
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-6 Receptor ELISA Kit (Interleukin-6 Receptor) 图像

  • Representative Standard Curve using ab46029

实验方案

Human IL-6 Receptor ELISA Kit (Interleukin-6 Receptor) (ab46029)参考文献

ab46029 has not yet been referenced specifically in any publications.

Product Wall

Abreview for IL6R Human ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Human Cell (lung cancer cell line)
Specification: lung cancer cell line
Amount of Sample: about 1,000,000 cells µg
Type of Kit Used: ELISA
Positive control: The control vials in the kit (430.9 pg/mL)
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Optimized incubation time of TMB substrate solution was 10 minutes in our laboratory. With15 minutes Incubation, the O.D. value of 1000 pg/mL standard reached over 2.5,
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Mrs. Hee Yi

Verified customer

提交于 May 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"