人IL-6 Receptor ELISA试剂盒(Interleukin-6 Receptor) (ab46029)

概述

  • 产品名称
    人IL-6 Receptor ELISA试剂盒(Interleukin-6 Receptor)
    参阅全部 IL6R 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 7 977.7pg/ml 3.04 0.31%
    Sample B 7 131.1pg/ml 2.489 2.289%
    批次间
    样品 n Mean SD CV%
    Sample A 24 983.9pg/ml 10.46 1.06%
    Sample B 24 136.2pg/ml 6.086 4.46%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 复检

    96 %

  • 检测时间
    1h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-6 Receptor (Interleukin-6 Receptor) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-6 Receptor in Human serum, plasma, buffered solutions and cell culture medium.


    A monoclonal antibody specific for IL-6 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 Receptor present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-6 Receptor.


    Get higher sensitivity in only 90 minutes with Human IL-6 Receptor ELISA Kit (ab181424) from our SimpleStep ELISA® range.


     

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL6R (CD126) Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-6 Receptor Microplate (12 x 8 well strips) 1 unit 2 units
    IL6R Control Silver 2 vials 4 vials
    IL6R Standard Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 功能
    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • 组织特异性
    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • 序列相似性
    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 结构域
    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • 翻译后修饰
    A short soluble form may also be released from the membrane by proteolysis.
  • 细胞定位
    Secreted and Basolateral cell membrane.
  • Information by UniProt
  • 别名
    • CD126
    • CD126 antigen
    • Gp80
    • IL 6R alpha
    • IL-6 receptor subunit alpha
    • IL-6R 1
    • IL-6R subunit alpha
    • IL-6R-1
    • IL-6R-alpha
    • IL-6RA
    • Il6r
    • IL6R1
    • IL6RA
    • IL6RA_HUMAN
    • IL6Ralpha
    • Interleukin 6 receptor
    • Interleukin-6 receptor subunit alpha
    • Membrane glycoprotein 80
    • MGC104991
    • OTTHUMP00000034320
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab46029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46029

实验方案

文献

This product has been referenced in:
  • Osuala KO  et al. Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration. BMC Cancer 15:584 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26268945) »

See 1 Publication for this product

客户评价及客户问答

Abreview for IL6R Human ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Human Cell (lung cancer cell line)
Specification: lung cancer cell line
Amount of Sample: about 1,000,000 cells µg
Type of Kit Used: ELISA
Positive control: The control vials in the kit (430.9 pg/mL)
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Optimized incubation time of TMB substrate solution was 10 minutes in our laboratory. With15 minutes Incubation, the O.D. value of 1000 pg/mL standard reached over 2.5,
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Mrs. Hee Yi

Verified customer

提交于 May 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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