人IL-6 ELISA试剂盒(Interleukin-6) (ab100573)

概述

  • 产品名称人IL-6 ELISA试剂盒(Interleukin-6)
    参阅全部 IL6 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    1.37 pg/ml - 1000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 93.65 82% - 102%
    Tissue Extracts 92.78 81% - 101%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-6 in cell lysates and tissue lysates.


    This assay employs an antibody specific for Human IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    For higher sensitivity in only 90 minutes, we recommend Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 疾病相关Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab100573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-6 ELISA Kit (Interleukin-6) 图像

  • Representative standard curve using ab100573

实验方案

Human IL-6 ELISA Kit (Interleukin-6) (ab100573)参考文献

ab100573 has not yet been referenced specifically in any publications.

Product Wall

Our lab used this kit in order to assess IL-6 levels in the supernatant of cultured Jurkat T cells. We used the following protocol for our culture:
1. Frozen Jurkat cells were thawed and washed in warm complete media (1640 RPMI + 10% FBS + antibiotic).
2. Cells were then allowed to rest in warm media for an hour.
3. Cells were then supplied with new media alone or containing an antioxidant (30 mins). Cells were then washed with 1X DPBS and proceeded to next step.
4. Cells from the two conditions in "3" were then supplied with complete media or complete media + 162 nM PMA (24 hours).---work was done in a 6 well plate (using only 4 wells). Basically the conditions were: 1) +antioxidant/+PMA, 2)+antioxidant/-PMA, 3)-antioxidant/+PMA, 4)-antioxidant/-PMA
5. Cells and media were separated by centrifugation (1000g, 5 mins) and used in the ELISA kit (ab100573).
6. Cells (after wash) were lysed with the kits supplied lysis buffer and cell extract (supernatant) was separated by centrifugation----This is an area in the kit that needs more clarification. There is absolutely no guide on how much of the lysis buffer to use (volume:cell ratio), incubation time, recommended temp, centrifugation speed, etc, which made using the lysis buffer to get cell extract complete guess work.
7. Cells and cell culture supernatant (media) were assayed at various dilutions (1:4, 1:5, and 1:6 for cells and undiluted, 1:2, and 1:4 for culture supernatant).
The kit was very easy to use as we've used similar kits by abcam before, but we saw very low levels of IL-6 for the culture supernatant at all dilutions (in some cases undetectable) while we saw IL-6 levels for cells across all dilutions (though we did not see any real difference across groups).
Overall, it seems the kit works with cell lysate, but not cell culture supernatant.
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提交于 Mar 09 2016

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The preparation for tissue using this kit is as follows.

2X lysis buffer is provided in the kit, and a protease inhibitor cocktail is recommended.

Choices of the method for lysis and homogeni...

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Thank you for your inquiry.
Do you have the catalogue numbers of the kits you're inquiring about? We have 7 different IL6 Human ELISA kits in the catalogue: ab46044, ab46042, ab46027, ab46041, ab100572, ab100573 and ab108867.
The IL6 High Sen...

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Thank you for your patience.

I've got great news - we have sourced the standards for these kits to be purchased separately! The catalog numbers for the standards will be:
IL1 beta human standard: ab134669
IL6 human recombinant st...

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Thank you for your enquiry.

As the on-line datasheets indictae ab100573 is suiatbel for cell culture extracts, tissue extracts, whilst ab100572 for cell culture supernatant, urine, serum, plasma.

I would advise you totake a look at te...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"