人IL-5 ELISA试剂盒(ab100571)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-5 ELISA试剂盒
参阅全部 IL-5 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 5 pg/ml -
范围
2.74 pg/ml - 2000 pg/ml -
回收率
95 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94.83 84% - 103% Serum 95.46 83% - 103% Plasma 96.32 84% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-5 2 vials IL-5 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
序列相似性
Belongs to the IL-5 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
see all -
数据库链接
- Entrez Gene: 3567 Human
- Omim: 147850 Human
- SwissProt: P05113 Human
- Unigene: 2247 Human
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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