人IL-4 Receptor ELISA试剂盒(CD124) (ab46022)

概述

  • 产品名称人IL-4 Receptor ELISA试剂盒(CD124)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 563.7pg/ml 17.8 3.1%
    Sample B 8 140.6pg/ml 9.6 6.8%
    批次间
    样品 n Mean SD CV%
    Sample A 8 530.5pg/ml 35.1 6.6%
    Sample B 8 106.5pg/ml 7.58 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 11.5 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 检测时间
    1h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human IL-4 Receptor (IL-4R) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-4 receptor molecule (CD124) in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4R present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-4R.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性IL4R binds interleukin 4 and interleukin 13 to regulate IgE production. It also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the IL4R can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene.
  • 细胞定位Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein
  • 别名
    • IL 4R alpha
    • IL4RA
    • ILR4A
    • Interleukin 4 receptor
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-4 Receptor ELISA Kit (CD124) 图像

  • Representative Standard Curve using ab46022

实验方案

Human IL-4 Receptor ELISA Kit (CD124) (ab46022)参考文献

ab46022 has not yet been referenced specifically in any publications.

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