人IL-33 ELISA试剂盒(Interleukin-33) (ab119547)

概述

  • 产品名称人IL-33 ELISA试剂盒(Interleukin-33)
    参阅全部 IL33 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.7%
    批次间
    样品 n Mean SD CV%
    Overall 6.9%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.2 pg/ml
  • 范围
    7.8 pg/ml - 500 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 73% - 92%
    Serum 67% - 79%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-33 (Interleukin-33) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-33 in cell culture supernatant, serum, and plasma (citrate, heparin).

    IL-33 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-33 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-33 captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.
  • 组织特异性Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • 序列相似性Belongs to the IL-1 family. Highly divergent.
  • 翻译后修饰Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C9orf26
    • DVS27
    • DVS27 related protein
    • IL-1F11
    • IL-33
    • IL1F11
    • IL33
    • IL33_HUMAN
    • Interleukin-1 family member 11
    • Interleukin-33
    • NF-HEV
    • NFEHEV
    • Nuclear factor from high endothelial venules
    • RP11-575C20.2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-33 ELISA Kit (Interleukin-33) 图像

  • Representative Standard Curve using ab119547.

实验方案

Human IL-33 ELISA Kit (Interleukin-33) (ab119547)参考文献

ab119547 has not yet been referenced specifically in any publications.

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