人IL-31 ELISA试剂盒(Interleukin-31) (ab119546)

概述

  • 产品名称
    人IL-31 ELISA试剂盒(Interleukin-31)
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.6%
    批次间
    样品 n Mean SD CV%
    Overall 2.5%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    1 pg/ml
  • 范围
    7.8 pg/ml - 500 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 115% - 123%
    Serum 86% - 106%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-31 (Interleukin-31) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-31 in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    IL-31 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an Biotin-conjugated anti-Human IL-31 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-31 captured on the plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
  • 组织特异性
    Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • IL-31
    • IL31
    • IL31_HUMAN
    • Interleukin-31
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-31 ELISA Kit (Interleukin-31) 图像

  • Representative Standard Curve using ab119546.

实验方案

Human IL-31 ELISA Kit (Interleukin-31) (ab119546)参考文献

ab119546 has not yet been referenced specifically in any publications.

Product Wall

very good Elisa

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We obtain reliable and reproducible results with this Elisa.
The protocol booklet is well explained, easy to understand and easy to perform.
I used this kit for serum samples from a comparison of healthy donor and atopic patients and and cell culture supernatants.
The kit is easy to use and quick compared to other Sandwich Elisa.
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Verified customer

提交于 Apr 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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