人IL-3 ELISA试剂盒(Interleukin-3) (ab100569)

概述

  • 产品名称
    人IL-3 ELISA试剂盒(Interleukin-3)
    参阅全部 IL3 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    16.46 pg/ml - 12000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 118 110% - 127%
    Serum 114.4 103% - 122%
    Plasma 98.82 86% - 118%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-3 (Interleukin-3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    700X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-3 2 vials
    IL-3 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-3 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
    This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
  • 组织特异性
    Activated T-cells, mast cells, natural killer cells.
  • 序列相似性
    Belongs to the IL-3 family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Burst promoting activity
    • Colony stimulating factor multiple
    • Eosinophil colony stimulating factor
    • Hematopoietic growth factor
    • IL 3
    • IL-3
    • IL3
    • IL3_HUMAN
    • Interleukin 3
    • Interleukin-3
    • Mast cell growth factor
    • MCGF
    • Multi CSF
    • Multilineage colony stimulating factor
    • Multipotential colony stimulating factor
    • Multipotential colony-stimulating factor
    • P cell stimulating factor
    • P-cell-stimulating factor
    • Thy1 inducing factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100569

  • Representative standard curve using ab100569

实验方案

文献

ab100569 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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