人IL-28A ELISA试剂盒(Interleukin-28A) (ab100567)

概述

  • 产品名称人IL-28A ELISA试剂盒(Interleukin-28A)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    13.72 pg/ml - 10000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 105.2 95% - 114%
    Serum 107 84% - 111%
    Plasma 110.4 84% - 120%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-28A (Interleukin 28A) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28A in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-28A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-28A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
  • 序列相似性Belongs to the IL-28/IL-29 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Cytokine ZCYTO20
    • EG330496
    • IFN-lambda-2
    • IFNL2
    • IL 28A
    • IL-28A
    • IL28A
    • IL28A_HUMAN
    • Interferon lambda 2
    • Interferon lambda-2
    • Interleukin 28A
    • Interleukin-28A
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-28A ELISA Kit (Interleukin-28A) 图像

  • Representative standard curve using ab100567

实验方案

Human IL-28A ELISA Kit (Interleukin-28A) (ab100567)参考文献

ab100567 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, we do not have any cross reactivity data with ab100567 and IL-28B. Since human IL-28A does have very high homology with human IL-28B (96%) it is possible that the antibody pair in the kit is also detecting IL-28B. However, without data t...

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Thank you for your patience.

Unfortunately, it looks like that neither the cross-reactivity has been determined, nor the epitope for this antibody mapped. However, as the two protein are up to 95% identical, it is very likely that the antibody...

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Thank you for your inquiry.

Unfortunately, epitope mapping has not been performed for the antibodies used in the ab100567, nor has IL-28B been tested with this kit so the cross reactivity with IL-28B is not known. Therefore this information w...

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Thank you for contacting us.

Unfortunately, we do not have any cross reactivity data of ab100567 with IL-28B. Since human IL-28A does have very high homology with human IL-28B (96%), it is possible that the antibody pair in the kit will also...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"