人IL-21 ELISA试剂盒(ab119542)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-21 ELISA试剂盒
参阅全部 IL-21 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 8 5.8% 批次间 样品 n Mean SD CV% Overall 8 7.7% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
20 pg/ml -
范围
78 pg/ml - 5000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 95 % - % Serum 71 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-21 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).
IL-21 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-21 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-21 captured on the plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human IL-21 monoclonal antibody 1 x 70µl Human IL-21 Standard lyophilized (10 ng/ml upon reconstitution) 2 vials Microplate coated with monoclonal antibody to Human IL-21 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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相关性
A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. IL-21 has been found to be a powerful growth factor for naive B cells. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAKSTAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
细胞定位
Secreted -
别名
- Cytokine CX2
- DN 452A22.7
- IL 21
see all -
数据库链接
- Entrez Gene: 59067 Human
- Omim: 605384 Human
- SwissProt: Q9HBE4 Human
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab119542 被引用在 5 文献中.
- Zhang Y et al. The Regulation between CD4+CXCR5+ Follicular Helper T (Tfh) Cells and CD19+CD24hiCD38hi Regulatory B (Breg) Cells in Gastric Cancer. J Immunol Res 2022:9003902 (2022). PubMed: 36339942
- Wang X et al. MicroRNA-146a-5p enhances T helper 17 cell differentiation via decreasing a disintegrin and metalloprotease 17 level in primary sjögren's syndrome. Bioengineered 12:310-324 (2021). PubMed: 33446013
- Liu Z et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
- Yamamoto M et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). PubMed: 29385874
- Zhang Z et al. Astragalus mongholicus (Fisch.) Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155. Front Pediatr 6:139 (2018). PubMed: 29977885