人IL-2 Receptor ELISA试剂盒(Interleukin-2 Receptor) (ab46036)

概述

  • 产品名称
    人IL-2 Receptor ELISA试剂盒(Interleukin-2 Receptor)
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.5%
    批次间
    样品 n Mean SD CV%
    1 6 1594pg/ml 184 11.6%
    2 6 1528pg/ml 135 8.8%
    3 6 1705pg/ml 204 12%
    4 6 1124pg/ml 171 15.2%
    5 6 2305pg/ml 165 7.2%
    6 6 1824pg/ml 126 69%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    32.5 pg/ml
  • 范围
    68.75 pg/ml - 2220 pg/ml
  • 复检

    87 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 99 86% - 122%

  • 检测时间
    3h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-2 Receptor (IL-2R) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2R in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Human IL-2R.

    A monoclonal antibody specific for IL-2R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2R present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-2 Receptor.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-2R Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-2R Control Silver 2 vials 4 vials
    IL-2R Microplate (12 x 8 well strips) 1 unit 2 units
    Plastic Plate Covers 2 units 4 units
    Stop Reagent Black 1 x 11ml 1 x 11ml
    Streptavidin-HRP 2 vials 4 vials
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性
    The IL2 Receptor, which is involved in T cell-mediated immune responses, alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Homodimeric beta (IL2RB) chains produce a medium-affinity receptor. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The interleukin 2 (IL2) receptor gamma chain (IL2RG), an important signalling component of many interleukin receptors (IL2,IL4,IL7,IL9, and IL15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked gene cause X-linked severe combined immunodeficiency (XSCID).
  • 细胞定位
    Cell Membrane; single-pass type I membrane protein.
  • 别名
    • CD122
    • CD122 antigen
    • CD132
    • CD132 antigen
    • CD25
    • CD25 antigen
    • IL2RA
    • IL2RB
    • IL2RG
    • IMD4
    • interleukin 2 receptor alpha
    • interleukin 2 receptor beta
    • interleukin 2 receptor gamma
    • p55
    • p64
    • p75
    • SCIDX
    • SCIDX1
    • TAC antigen
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46036

实验方案

文献

This product has been referenced in:
  • Ngcobo M & Gqaleni N Evaluation of the immunomodulatory effects of a South African commercial traditional immune booster in human peripheral blood mononuclear cells. BMC Complement Altern Med 16:300 (2016). Read more (PubMed: 27550057) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us. The two soluble IL2 receptor ELISA kits, ab46036 (with plate) and ab47354 (capture and detection antibodies sufficient for five plates, but plates are not included) are known to be reactive with the alpha sub-unit. Reactivi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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