人IL-2 ELISA试剂盒(Interleukin-2) High Sensitivity (ab46054)

概述

  • 产品名称人IL-2 ELISA试剂盒(Interleukin-2) High Sensitivity
    参阅全部 IL2 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5 4.2%
    批次间
    样品 n Mean SD CV%
    Overall 5 9%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.97 pg/ml
  • 范围
    1.87 pg/ml - 60 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95 % - %
    Serum 100 % - %

  • 检测时间
    2h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human High Sensitivity IL-2 ELISA is to be used for the in-vitro quantitative determination interleukin-2 (IL-2) in Human sera, plasmas, buffered solutions or cell culture media.

    A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-2.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 疾病相关Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • 序列相似性Belongs to the IL-2 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-2 ELISA Kit (Interleukin-2) High Sensitivity 图像

  • Representative standard curve using ab46054

实验方案

Human IL-2 ELISA Kit (Interleukin-2) High Sensitivity (ab46054)参考文献

ab46054 has not yet been referenced specifically in any publications.

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