人IL-1a ELISA试剂盒(Interleukin-1 alpha) (ab46028)

概述

  • 产品名称
    人IL-1a ELISA试剂盒(Interleukin-1 alpha)
    参阅全部 IL1 alpha 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 22 459.8pg/ml 13 2.8%
    Sample B 21 72.6pg/ml 3.9 4.3%
    批次间
    样品 n Mean SD CV%
    Sample A 8 259.9pg/ml 15 5.8%
    Sample B 8 58.6pg/ml 4.3 7.3%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    31.2 pg/ml - 1000 pg/ml
  • 复检

    95.3 %

  • 检测时间
    3h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-1a (Interleukin-1 alpha) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1a in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.


    A monoclonal antibody specific for IL-1a has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1a concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1a to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1a is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample. 


    This kit will recognize both endogenous and recombinant Human IL-1a.


    Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.


     

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL1a Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL1a Control Silver 2 vials 4 vials
    IL-1a Microplate (12 x 8 well strips) 1 unit 2 units
    IL-1a Standard (Lyophilized) Yellow 2 vials 4 vials
    Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性
    IL1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
  • 细胞定位
    Secreted
  • 别名
    • Hematopoietin 1
    • IL 1 alpha
    • Il 1a
    • IL1
    • IL1 ALPHA
    • IL1A
    • IL1F1
    • Ilia
    • Interleukin 1 alpha
    • Interleukin 1 alpha precursor
    • Interleukin1 alpha
    • OTTHUMP00000203692
    • OTTMUSP00000016430
    • Preinterleukin 1
    • Pro interleukin 1
    • Pro interleukin 1 alpha
    • Prointerleukin 1
    • RP23-160G19.8
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab46028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46028

实验方案

文献

ab46028 has not yet been referenced specifically in any publications.

客户评价及客户问答

This information is provided in the Protocol Booklet (pdf file) which can be found and down-loaded from the datasheet (top right corner). Please see section Assay Procedure 13.14:

"Read absorbance of each well on a spectrophotometer usin...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册