人IL-1a ELISA试剂盒(Interleukin-1 alpha) (ab46028)

概述

  • 产品名称人IL-1a ELISA试剂盒(Interleukin-1 alpha)
    参阅全部 IL1 alpha 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 22 459.8pg/ml 13 2.8%
    Sample B 21 72.6pg/ml 3.9 4.3%
    批次间
    样品 n Mean SD CV%
    Sample A 8 259.9pg/ml 15 5.8%
    Sample B 8 58.6pg/ml 4.3 7.3%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    31.2 pg/ml - 1000 pg/ml
  • 复检

    95.3 %

  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-1a (Interleukin-1 alpha) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1a in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.

    A monoclonal antibody specific for IL-1a has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1a concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1a to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1a is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample. 

    This kit will recognize both endogenous and recombinant Human IL-1a.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性IL1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
  • 细胞定位Secreted
  • 别名
    • Hematopoietin 1
    • IL 1 alpha
    • Il 1a
    • IL1
    • IL1 ALPHA
    • IL1A
    • IL1F1
    • Ilia
    • Interleukin 1 alpha
    • Interleukin 1 alpha precursor
    • Interleukin1 alpha
    • OTTHUMP00000203692
    • OTTMUSP00000016430
    • Preinterleukin 1
    • Pro interleukin 1
    • Pro interleukin 1 alpha
    • Prointerleukin 1
    • RP23-160G19.8
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-1a ELISA Kit (Interleukin-1 alpha) 图像

  • Representative Standard Curve using ab46028

实验方案

Human IL-1a ELISA Kit (Interleukin-1 alpha) (ab46028)参考文献

ab46028 has not yet been referenced specifically in any publications.

Product Wall

This information is provided in the Protocol Booklet (pdf file) which can be found and down-loaded from the datasheet (top right corner). Please see section Assay Procedure 13.14:

"Read absorbance of each well on a spectrophotometer usin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"