人IL-18 R beta ELISA试剂盒(Interleukin-18 R beta) (ab113325)

概述

  • 产品名称人IL-18 R beta ELISA试剂盒(Interleukin-18 R beta)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 12 pg/ml
  • 范围
    12.3 pg/ml - 3000 pg/ml
  • 复检

    77 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 75.78 68% - 85%
    Serum 80.9 71% - 89%
    Plasma 74.98 67% - 83%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-18 R beta (Interleukin-18 R beta) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.
  • 组织特异性Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.
  • 序列相似性Belongs to the interleukin-1 receptor family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • IL18RAP
    • Accessory protein like
    • Accessory protein-like
    • AcPL
    • CD218 antigen-like family member B
    • CD218b
    • CDw218b
    • I18RA_HUMAN
    • IL 18RAcP
    • IL 1R accessory protein like
    • IL 1R7
    • IL 1RAcPL
    • IL-18 receptor accessory protein
    • IL-18R-beta
    • IL-18RAcP
    • IL-18Rbeta
    • IL-1R accessory protein-like
    • IL-1R-7
    • IL-1R7
    • IL-1RAcPL
    • IL18RAP
    • IL18Rbeta
    • Interleukin 18 receptor accessory protein
    • Interleukin-1 receptor 7
    • Interleukin-18 receptor accessory protein
    • Interleukin-18 receptor accessory protein-like
    • Interleukin-18 receptor beta
    • Interleukin-18 receptor beta chain
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-18 R beta ELISA Kit (Interleukin-18 R beta) 图像

  • Representative Standard Curve using ab113325

实验方案

Human IL-18 R beta ELISA Kit (Interleukin-18 R beta) (ab113325)参考文献

ab113325 has not yet been referenced specifically in any publications.

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