人IL-18 BP ELISA试剂盒(Interleukin-18 Binding Protein) (ab100559)

概述

  • 产品名称人IL-18 BP ELISA试剂盒(Interleukin-18 Binding Protein)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    91 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 106.7 96% - 121%
    Serum 83.5 74% - 97%
    Plasma 82.3 72% - 96%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-18 BP (Interleukin-18 Binding Protein) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 BP in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-18 BP coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18 BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
  • 组织特异性Strongly expressed in heart, lung, placenta and spleen.
  • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • I18BP_HUMAN
    • IL-18BP
    • IL18 BP
    • IL18 BPa
    • IL18BP
    • IL18BPa
    • Interleukin 18 binding protein
    • Interleukin-18-binding protein
    • MC51L 53L 54L homolog gene product
    • Tadekinig alfa
    • Tadekinig-alfa
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-18 BP ELISA Kit (Interleukin-18 Binding Protein) 图像

  • Representative standard curve using ab100559

  • Representative standard curve using ab100559

实验方案

Human IL-18 BP ELISA Kit (Interleukin-18 Binding Protein) (ab100559)参考文献

ab100559 has not yet been referenced specifically in any publications.

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