人IL-18 BP ELISA试剂盒(Interleukin-18 Binding Protein) (ab100559)

概述

  • 产品名称
    人IL-18 BP ELISA试剂盒(Interleukin-18 Binding Protein)
    参阅全部 IL18 binding protein 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    91 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 106.7 96% - 121%
    Serum 83.5 74% - 97%
    Plasma 82.3 72% - 96%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-18 BP (Interleukin-18 Binding Protein) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 BP in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-18 BP coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18 BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100559

  • Representative standard curve using ab100559

实验方案

文献

This product has been referenced in:
  • Menale C  et al. Bisphenol A effects on gene expression in adipocytes from children: association with metabolic disorders. J Mol Endocrinol 54:289-303 (2015). Read more (PubMed: 25878060) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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