人IL-17A/F ELISA试剂盒(without plates) (ab105137)

概述

  • 产品名称人IL-17A/F ELISA试剂盒(without plates)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 9 230.3pg/ml 8.1 3.5%
    B 9 313.4pg/ml 19.2 6.1%
    C 9 435.5pg/ml 32.4 7.5%
    批次间
    样品 n Mean SD CV%
    A 9 230.1pg/ml 9.7 4.2%
    B 9 313.2pg/ml 20.1 6.4%
    C 9 435.3pg/ml 31.3 7.2%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 7 pg/ml
  • 范围
    15.6 pg/ml - 500 pg/ml
  • 复检

    107 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 102 77% - 117%

  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-17A + IL-17F Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A + IL-17F in supernatants, buffered solutions or serum and plasma samples and other body fluids.

    A monoclonal antibody specific for IL-17A + IL-17F has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17A + IL-17F concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17A + IL-17F are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17A + IL-17F present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-17A + IL-17F.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性IL17A induces stromal cells to produce proinflammatory and hematopoietic cytokines and enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. Il17F stimulates the production of other cytokines such as IL6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. It also stimulates PBMC and T-cell proliferation and inhibits angiogenesis.
  • 细胞定位Secreted
  • 别名
    • C87042
    • CTLA8
    • Cytokine ML 1
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein
    • Cytotoxic T lymphocyte associated serine esterase 8
    • IL 17
    • IL 17A
    • IL 17F
    • IL 24
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • Interleukin 17F
    • Interleukin 24
    • MGC114402
    • ML 1
    • ML1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab105137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-17A/F ELISA Kit (without plates) 图像

  • Representative standard curve using ab105137

实验方案

Human IL-17A/F ELISA Kit (without plates) (ab105137)参考文献

ab105137 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"