人IL-17A ELISA试剂盒(Interleukin-17A) Receptor ELISA试剂盒(ab100558)

概述

  • 产品名称人IL-17A ELISA试剂盒(Interleukin-17A) Receptor ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    12.35 pg/ml - 3000 pg/ml
  • 复检

    81 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 81.54 70% - 92%
    Serum 87.64 83% - 104%
    Plasma 73.27 67% - 87%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-17A (Interleukin 17A) Receptor Human ELISA (Enzyme Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17A in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling.
  • 组织特异性Widely expressed.
  • 疾病相关Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5) [MIM:613953]. CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • 序列相似性Contains 1 SEFIR domain.
  • 翻译后修饰Glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD217
    • CDw217
    • hIL 17R
    • I17RA_HUMAN
    • IL 17 receptor A
    • IL 17RA
    • IL-17 receptor A
    • IL-17RA
    • IL17R
    • IL17RA
    • Interleukin 17 receptor A
    • Interleukin-17 receptor A
    • MGC10262
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human IL-17A ELISA Kit (Interleukin-17A) Receptor ELISA Kit 图像

  • Representative standard curve using ab100558

  • Representative standard curve using ab100558

实验方案

Human IL-17A ELISA Kit (Interleukin-17A) Receptor ELISA Kit (ab100558)参考文献

ab100558 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and interest in our product. I can confirm that this kit (Ref: ab100558) was not validated specifically with BAL samples - only with serum, plasma and cell culture supernatants. As long as the BAL samples have detectabl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"