人IL-17a ELISA试剂盒(Interleukin -17a) (ab83688)

概述

  • 产品名称
    人IL-17a ELISA试剂盒(Interleukin -17a)
    参阅全部 IL-17a 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 6 3.3%
    批次间
    样品 n Mean SD CV%
    Overall 6 5.2%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    3.12 pg/ml - 100 pg/ml
  • 复检

    105 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 121 113% - 130%

  • 检测时间
    3h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s IL-17a (Interleukin-17a) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative of IL-17a in supernatants, buffered solutions, serum, plasma and other body fluids.


    A monoclonal antibody specific for IL-17a has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17a concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17a are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17a present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-17a.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab83688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • IL-17 measured in cell culture supernatants (dilution range 1/1-1/100) from control (PBMC) or treated ((PBMC+PHA) 48 hours; 2% PHA-M; LifeTechnologies) cells (duplicates +/- SD).

  • Representative Standard Curve using ab83688

实验方案

文献

This product has been referenced in:
  • Yu X  et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . Read more (PubMed: 22174866) »

See 1 Publication for this product

客户评价及客户问答

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He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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